|
|
Barley Proteomic Studies Related to Beer Production
Benkovská, Dagmar ; Márová, Ivana (referee) ; Ehrenbergerová, Jaroslava (referee) ; Zdráhal, Zbyněk (referee) ; Bobáľová, Janette (advisor)
Tato práce se zabývá proteomickými studiemi ječmene v souvislosti s výrobou piva. Ječmen patří mezi nejvýznamnější plodiny na světě a je využíván hlavně pro sladovnické účely, nejčastěji pro pivovarnictví. Studium proteinů ječmene během sladování a výroby piva poskytuje informace o změnách v proteinovém složení nebo jejich posttranslačních modifikacích. Jelikož jsou proteiny v ječmeni a jejich změny zásadní pro kvalitu sladu a piva, proteomické studie ječmene mají potenciál pro zlepšení procesu sladování a pivovarnictví. Hlavním cílem této práce je studium ve vodě rozpustných proteinů ječmene a jejich změn, ke kterým dochází během sladování a výroby piva. Rozdíly v proteinovém složení byly sledovány pomocí gelové elektroforézy, kapalinové chromatografie na reverzní fázi, gelové chromatografie a MALDI-TOF hmotnostní spektrometrie. Během sladování se vlivem klíčení zrna zvyšuje množství některých proteinů a také jsou tvořeny nové proteiny. V průběhu vaření piva se naopak v důsledku vysoké teploty a enzymatické aktivity proteáz mnoho proteinů rozkládá. Těmto drsným podmínkám odolají jen některé proteiny, které přechází až do piva a mohou ovlivnit jeho kvalitu. Dále byly zkoumány různé odrůdy ječmene a jejich rozdíly. Byly porovnány odrůdy povolené pro výrobu certifikovaného Českého piva s jednou osvědčenou sladovnickou odrůdou a jednou nesladovnickou odrůdou ječmene. Kromě toho byly studovány v alkoholu rozpustné proteiny ječmene a jejich změny v průběhu sladování. Zvláštní pozornost byla věnována vybrané skupině posttranslačních modifikací proteinů: glykosylacím. Neenzymaticky glykosylované proteiny ječmene (neboli glykované proteiny) jsou tvořeny v průběhu sladování kvůli přítomnosti velkého množství glukózy uvolněné z rozkladu škrobu. Glykované proteiny ovlivňují stabilitu proteinů a kvalitu piva, obzvlášť pěnotvorný účinek. Enzymatické N-glykosylace představují nejčastěji studované posttranslační modifikace u rostlin, protože glykoproteiny hrají klíčovou roli v různých biologických funkcích. Glykoproteiny jsou často přítomny v malém množství, a proto je pro jejich analýzu potřebné obohacení glykoproteinů z komplexní směsi. Pro studium glykoproteinů byla využita afinitní chromatografie s lektinem concanavalin A. Kromě toho byla také optimalizována analýza sacharidové části glykoproteinů. Tato disertační práce přináší důležité informace o proteinech ječmene, jejich změnách a analýze, které budou užitečné pro další studium.
|
|
|
Analysis of low-molecular proteins in barley by the SDS-PAGE method during malting
Myslivcová, Pavla ; Svoboda, Zdeněk (referee) ; Mikulíková, Renata (advisor)
This diploma thesis deals with the analysis of low molecular weight proteins in barley during malting by SDS-PAGE method. Attention was paid to PR proteins, specifically LTP proteins and thionins, considered to be connected with gushing effect. Barley samples, malt intermediates and malt samples taken in 10 consecutive days to cover the whole malting process were used for the experiment. In total, 5 samplings were used for the analysis. Proteins extracted from the samples were separated by SDS-PAGE using a Tris-tricine buffer system. The protein lines of LTP proteins and thionins were identified in the resulting gels. The relative optical density values of the selected protein bands were obtained to assess the effect of malting on these proteins. A similar pattern of change in the content of mentioned low molecular weight proteins during the malting process was observed. This was confirmed by finding a statistically significant positive correlation between the relative optical density values of LTP proteins and thionins. Furthermore, the relationship between the low molecular weight protein content and the gushing potential and the microbiological contamination of the samples was investigated, but was not confirmed.
|
|
|
Proposal of Work-Life Balance in Selected Company
Zavadilová, Lada ; Nováková, Petra (referee) ; Rašticová, Martina (advisor)
The diploma thesis deals with the issue of work-life balance of employees and especially managers in a chosen company. The theoretical part is about issues of gender, factors influencing managers’ careers and possibilities of work-life balance. In the practical part, there is an analysis of a present situation in a company. On the basis of theoretical findings, analysis of company’s documents, a questionnaire and interviews with chosen employees, there will be made some suggestions for improving a present situation in a company. There will be also a financial evaluation of new steps as a part of final suggestion.
|
|
|
The Proposal of Work-Life Balance in Selected Company
Švejdíková, Romana ; Mikyska, Martin (referee) ; Rašticová, Martina (advisor)
The diploma thesis deals with the issue of work-life balance in a chosen organisation. The work life balance instruments are described in detail and theoretical findings are compared with reality. The thesis identifies possibilities and barriers to work-life balance of employees of the observed company. On the basis of semistructured interview and questionnaire survey, measures for optimal work life balance are suggested.
|
|
|
Harmonisation of work and family life - the perspective of Czech and European law
Fantová, Karolína ; Koldinská, Kristina (advisor) ; Munková, Gabriela (referee)
Harmonisation of work and family life - the perspective of Czech and European law Harmonising work and family life is a very topical issue today. Employees no longer want to choose between work and care; they want the possibility to do both. The legal framework responds to this need - it creates conditions that support this harmonisation. This has a positive impact on productivity and employee satisfaction. Moreover, balancing work and family life positively influences societal factors, such as demographic stability and sustainable economic growth, making it a politically intriguing topic as well. The thesis offers a comprehensive view of the work and family life harmonisation in the context of the current legislation in the Czech Republic and within the European Union, focusing especially on caregivers. The main goal of the thesis is to provide readers with a comprehensive overview of current legislation concerning the harmonisation of work and family life, particularly focusing on flexible forms of employment, obstacles at work from the employee's side, and financial security in situations where the employee cannot work due to caring for another person. The thesis further examines European law standards and their impact on domestic legislation, noting also the non-legislative initiatives of the...
|
|
|
The comparison of Czech and German family policy enabling the harmonization of work and family life
Estočinová, Barbora ; Dobiášová, Karolína (advisor) ; Vlčková, Kamila (referee)
The subject of this thesis is the comparison of family policy in the Czech Republic and Federal Republic of Germany in terms of measures adjustment enabling the reconciliation of work and care about the preschool children. The issue of work-life balance is further presented in the context of traditional sociological concepts and welfare state model theories, followed by a description of trends and current order of women's employment on the labor market of both countries and accompanied by the description of demographic trends. Thesis is specifically focused on the description of the governmental family policy of both countries in the perspective of parental leave entitlement settings, facilities of children day care facilities like nursery schools and creche, and formal involvement of fathers in the childcare. The methodological part of thesis is focused on the description of the family policy measures of the respective country. Comparison of both countries in terms of enabling the reconciliation of work and childcare is made on the basis of analyse and comparison of both the secondary quantitative data and the qualitative data drawn from the discussions placed on the free available web sites. The conclusion of thesis tackles the question of possible applicability of the family policy measures in...
|
|
|
Work - life balance
Mýtinová, Melissa Marie ; Tomšej, Jakub (advisor) ; Koldinská, Kristina (referee)
in English language Work-life balance This diploma thesis explores various (international, European, and Czech) legal sources and practices related to work-life balance. The changes brought by the Directive (EU) 2019/1158 of the European parliament and of the Council of 20 June 2019 on work-life balance for parents and carers are also taken into account throughout the thesis. The historical development of working time is analysed, as well as its current status in modern society and expectations for the future. Different forms of flexible working arrangements, such as flexible working hours, job-sharing, and homeworking, are discussed in detail, along with the benefits and drawbacks of each. The thesis also covers part-time jobs and their role in promoting work-life balance. The role of obstacles to work on the employee's side in balancing their personal and work life is also described, particularly those related to life events such as childbirth and caring for children or close family members. This includes maternity leave, paternity leave, parental leave, carer's leave (caring for a family or household member) and long-term care. The related financial compensations that correspond to these obstacles to work are also described. The thesis further provides information on other tools for balancing...
|
|
|
Reconciliation of family and work life in healthcare
Mandíková, Marie ; Štangová, Věra (advisor) ; Tomšej, Jakub (referee)
Reconciliation of family and work life in healthcare Abstract Reconciliation of family and work life is currently a topic of fundamental importance, not only from the perspective of the individual, but also from the perspective of society, as it relates to many issues of societal importance, such as the promotion of equality between men and women on the labour market, increasing the employment of women and motivating young couples to start families in order to increase the birth rate. Although the topic of harmonization of work and family life is currently receiving relatively high attention, and the regulations of labour law and social security law contain a wide range of instruments supporting the reconciliation of family and work life, it shall be remembered that there are professions that are more accessible than others for harmonizing work and family life (especially, for instance, "white collar" jobs, with the possibility of relatively wide flexibility). However, there are also professions in which it is very difficult to imagine an employee working from home or using, for instance, flexible working hours, as the nature of that particular profession does not allow it. This diploma thesis is devoted to the conflict between the legal theory of the harmonization of family and work life with practice in a...
|
|
|
Reconciling personal and work life
Staňo, Pavel ; Hůrka, Petr (advisor) ; Morávek, Jakub (referee)
Reconciling personal and work life represents one of the modern trends in the subject of the labour law. The purpose of this area is to offer employees more flexibility in labour relations in favour of their personal lives. The search for the optimal point of balance in personal and work life of an employee is getting to the forefront. In the discussions this subject is more and more popular and became an important topic in the public space not only in the Czech Republic, but also in other European countries, or on the grounds of the European Union institutions. This, combined with the needs and interests of the employers to maintain a functioning and competitive economy, represents the fundamental theme of this thesis. Setting up the system to suit both employees and employers represents a big challenge which is still waiting to be solved. This thesis points out in this area the possibilities of current legislature with their limits and offers potential future direction, because current legislature is insufficient in this area. This thesis offers to the reader insight into current legislation in reconciling personal and work life, primarily through individual institutes of current labour law including basic historical context. This thesis explains the description of individual institutes, shows...
|
|
|
Harmonization of Family and Professional Life of Female Doctors in the Czech Republic
Handzelová, Juliána ; Hasmanová Marhánková, Jaroslava (advisor) ; Tučková, Anna (referee)
This bachelor thesis deals with the harmonization of family and professional life of female doctors in the Czech Republic. The topic is explored through a gender perspective. The aim of the thesis is to determine how women doctors feel during their study years and subsequently during their professional life, whether they have ever encountered gender discrimination, how female doctors manage to reconcile their family and professional life, and what obstacles they are faced with in such a demanding profession. The thesis describes the medical profession and its genderalization, family and parenthood, the public and private spheres, the harmonization of family and professional life, and what strategies can be used for its reconciliation. The methodological approach is qualitative with a research design of a case study. To answer the research questions, semi-structured interviews with female doctors who are working in hospitals and have at least one child under the age of 13 (including) were conducted. The outcomes of the analysis show the existence of gender discrimination and sexism, describe the attestation system that is unfavorable towards women, outline what female doctors are confronted with when reconciling family and professional life and in what ways does having a family affect their...
|
guest ::
